The Lidstrom group provides biological expertise for the Track II team. In addition, microwell array chips are being developed with smaller, more densely packed arrays that can be imaged at higher magnifications.

Group Members

Mary Lidstrom
Jungers Professor Chemical Engineering, University of Washington
Professor of Microbiology, University of Washington
Director, Microscale Life Sciences Center
Vice Provost for Research, University of Washington
lidstrom@u.washington.edu
452 Benjamin Hall Interdisciplinary Research Building

Linda Sauter
Graduate student (Microbiology/Astrobiology)
lmsauter@u.washington.edu
443 Benjamin Hall Building Interdisciplinary Research

CLONAL EVOLUTION IN THE HUMAN PREMALIGNANT CONDITION, BARRETT'S ESOPHAGUS; HUMAN SOMATIC GENOMIC ANALYSIS, IN VIVO.

Barrett's esophagus is a premalignant condition that develops as a complication of chronic gastroesophageal reflux in which the normal squamous epithelium of the esophagus is replaced by a metaplastic columnar epithelium. We are studying a large cohort of patients with Barrett's esophagus in whom serial biopsies are being taken prospectively to evaluate genomic and expression abnormalities that predict future progression to cancer. We are using high-throughput and comprehensive technologies, including fluorescent genotyping and sequencing as well as SNP and methylation arrays to investigate neoplastic evolution in humans in vivo to better understand the clonal dynamics that result in cancer versus a benign outcome. The Barrett's Project is a multidisciplinary scientific investigation in which laboratory, clinical and population scientists collaborate to elucidate human biological mechanisms that predispose to cancer while simultaneously using this knowledge in clinical and cohort studies to develop interventions to prevent or treat esophageal adenocarcinoma and its precursors.

Group Members

Brian J. Reid, MD, PhD
Member, Human Biology and Public Health Sciences Divisions
Fred Hutchinson Cancer Research Center
Professor of Medicine, Adjunct Professor of Genome Sciences
University of Washington
bjr@fhcrc.org

David Hockenbery, MD
Member, Clinical Research and Human Biology
Fred Hutchinson Cancer Research Center
Professor, School of Medicine, Medicine, Gastroenterology, University of Washington
dhockenb@fhcrc.org

Thomas Paulson, PhD
Staff Scientist
Seattle Barrett's Esophagus Program
Fred Hutchinson Cancer Research Center
tpaulson@fhcrc.org

Carissa (Rissa) Sanchez,
Research Project Manager
Seattle Barrett's Esophagus Program
Fred Hutchinson Cancer Research Center
rissa@fhcrc.org

Martin Suchorolski
PhD Graduate Student
Seattle Barrett's Esophagus Program
mtsuchor@yahoo.com

Judy Anderson
Research Technician
Peter Rabinovitch Laboratory
University of Washington
jbanders@u.washington.edu

Group Lead: Norman J. Dovichi

The Proteomics Group is developing tools to study the proteome with two-dimensional capillary electrophoresis and laser-induced fluorescence. This group's long-term goal is to study protein expression in single cells and to determine how protein expression changes across a cellular population during cancer progression, proinflammatory cell death, and during the development of an embryo. This group works closely with the Neoplastic Progression Group, using Barrett's esophagus as a model to study the heterogeneity in protein expression during cancer progression.

Group Members

Norm Dovichi
Department of Chemistry
University of Washington
Seattle, WA 98195-1700 USA
e-mail: dovichi@chem.washington.edu
Web page: http://faculty.washington.edu/dovichi

Jane Dickerson
Graduate student (Chemistry)
Janed4@u.washington.edu

Kristian Swearingen
Graduate student (Chemistry)
keschem@u.washington.edu

Rosa Wojcik
Graduate student (Chemistry)
rozaw@u.washington.edu